Larvae were transferred to new cultures each day and fed on newly-hatched Artemia nauplii. While transferring, larvae were checked for mortality and sorted by developmental stage. At high temperatures (30 and 35 ºC) the cultures were covered with adhesive plastic to prevent evaporation and consequent salinity increase. Theexperiment lasted until the juvenile stage was reached.
Temperature and salinity effects on survival of larval stages were tested by 2-way factorial ANOVA. Percent cumulative survival was transformed by angular transformation (arcsin √proportion). Regression coefficients were used in the polynomial expression to generate a surface response contour (Box & Youle, 1955):
Z = b0+ b1 T + b2 S + b11T + b22S + b12TS
where Z is the surface response, T is temperature, S is salinity, b0 is the multiple regression constant, b1 and b2 are the linear effects of temperature and salinity, b11 and b22 are the quadratic effects, and b12 is the intersection effect. No statistical test was applied to duration of larval stages, as total mortality in a number of extreme temperature and salinity combinations makes the planned comparisons highly unbalanced and of restricted interpretation value.
Larvae were transferred to new cultures each day and fed on newly-hatched Artemia nauplii. While transferring, larvae were checked for mortality and sorted by developmental stage. At high temperatures (30 and 35 ºC) the cultures were covered with adhesive plastic to prevent evaporation and consequent salinity increase. Theexperiment lasted until the juvenile stage was reached.
Temperature and salinity effects on survival of larval stages were tested by 2-way factorial ANOVA. Percent cumulative survival was transformed by angular transformation (arcsin √proportion). Regression coefficients were used in the polynomial expression to generate a surface response contour (Box & Youle, 1955):
Z = b0+ b1 T + b2 S + b11T + b22S + b12TS
where Z is the surface response, T is temperature, S is salinity, b0 is the multiple regression constant, b1 and b2 are the linear effects of temperature and salinity, b11 and b22 are the quadratic effects, and b12 is the intersection effect. No statistical test was applied to duration of larval stages, as total mortality in a number of extreme temperature and salinity combinations makes the planned comparisons highly unbalanced and of restricted interpretation value.
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