Background:
The rapid evolution of 454 GS-FLX sequencing technology has not been accompanied by a
reassessment of the quality and accuracy of the sequences obtained Current strategies for decision-making and
error-correction are based on an initial analysis by Huse
et al.
in 2007, for the older GS20 system based on
experimental sequences. We analyze here the quality of 454 sequencing data and identify factors playing a role in
sequencing error, through the use of an extensive dataset for Roche control DNA fragments