Chloroform was removed with a rotary evaporator under vacuum. Distilled water (100 ml) was added to the ß-carotene emulsion and mixed well. 3 ml aliquot of the ß-carotene emulsion and
0.2 ml of the diluted extract were placed in a test tube and mixed
well which was immediately incubated in water bath at 50 C.
Sample absorbance was measured at 470 nm (60 min after incubation) against control.