Preparation of potato flour slurry
Slurries of various concentrations (10, 15, 20, 25 and 30% w/v) of potato flour starch was prepared in water and treated with liquefying enzyme (0.15% v/w) at 104 to 105°C for 60 min in an autoclave. The slurry prepared by mixing 25 g flour in 100 ml water (1:4) being homogenous, loose, easy to handle was used for further experi- ments. Liquefaction of potato flour (100 ml slurry) was carried out at 104 to 105°C in an autoclave using varying concentration of alpha amylase (0.05 to 0.20% v/w) for different time intervals (10 to 240 min). The progress of liquefaction was monitored by employing starch-iodine (1 g of iodine and 2 g KI in 100 ml water) reaction. Saccharification of liquefied starch was carried out at 60°C for different time intervals using varying concentration (0.05 to 0.45% v/w) of amyloglucosidase. The reaction was monitored by the yield of total reducing sugars estimated by dinitrosalicylic acid method (Miller, 1959).