30 mL aliquots were mixed with 90 mL of distilled water and 900 mL of FRAP re- agent, and the absorbance of the reaction mixture was measured spectrophotometrically at 593 nm following incubation at 37 C for
2 h. The FRAP concentration was calculated from a calibration curve obtained by linear regression. The results are expressed in Trolox activity equivalents (mmoles TE g 1 seed)