Methods
We interviewed key informants (health centre staff, hosts of the wedding party, some persons involved in food preparation and a few patients). A list of party attendees and the menu were obtained. Wedding attendees came from eight provinces, and at the time of the field investigation many attendees had returned to their home provinces. We were therefore only able to interview attendees who lived in the village where the wedding was held and a nearby village. A team of eight persons interviewed these wedding party attendees in person using a standard foodborne illness questionnaire. Data on demographic information, signs and symptoms, onset of illness, time of meal and food items consumed during the party were obtained.
Since we were only able to interview 27% of the guests (69/256), we used a case-control study design. Cases were wedding party attendees who developed acute diarrhoea (> three loose bowel movements in a day) after the party anytime from 8 to 10 April. Controls were wedding party attendees who remained well during the three days after the party.
We obtained rectal swabs from 13 cases who agreed to have samples taken from them. The samples were sent to the laboratory at the National Institute for Public Health, Cambodia for bacterial culture.
Data were entered into a computer and analysed using Epi Info. We calculated odds ratios, confidence intervals and P values using the Fischer's exact test (2-tailed) and Yates corrected chi-squared test.
MethodsWe interviewed key informants (health centre staff, hosts of the wedding party, some persons involved in food preparation and a few patients). A list of party attendees and the menu were obtained. Wedding attendees came from eight provinces, and at the time of the field investigation many attendees had returned to their home provinces. We were therefore only able to interview attendees who lived in the village where the wedding was held and a nearby village. A team of eight persons interviewed these wedding party attendees in person using a standard foodborne illness questionnaire. Data on demographic information, signs and symptoms, onset of illness, time of meal and food items consumed during the party were obtained.Since we were only able to interview 27% of the guests (69/256), we used a case-control study design. Cases were wedding party attendees who developed acute diarrhoea (> three loose bowel movements in a day) after the party anytime from 8 to 10 April. Controls were wedding party attendees who remained well during the three days after the party.We obtained rectal swabs from 13 cases who agreed to have samples taken from them. The samples were sent to the laboratory at the National Institute for Public Health, Cambodia for bacterial culture.Data were entered into a computer and analysed using Epi Info. We calculated odds ratios, confidence intervals and P values using the Fischer's exact test (2-tailed) and Yates corrected chi-squared test.
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