The seeds showed differential germination percentages in different basal media and maximum germination of seeds occurred in PM medium only. Peptone supported better growth and survival (∼100 %) of protocorms.
Tulasnella fungi are a major group of mycorrhizal fungi forming symbiotic association with orchids in the tropics.
Tulasnella supported germination and seedling development,
but Trichoderma, also obtained from D. aphyllum using seed baiting techniques, had a negative effect on germination.
Tulasnella isolates (JC-02 and JC-05) showed a significant, positive effect on seeds germination of D. officinale.