The variables MgSO4 and agitation rate have a significant
effect on tannase production (p < 0.1), and these
variables were selected for the CCRD. MgSO4 has shown
a positive effect on enzyme production, indicating that an
increase in this compound in the culture medium can
favor enzyme production. The agitation rate showed a
significant negative effect, which indicates that lower
agitation levels yield higher levels of tannase production.
The salt solution was not part of the initial composition
of the culture medium and was removed from the medium
for the next step to minimize the enzyme production
costs