The differential pulse anodic stripping voltammetry method was used for the determination of zinc released into artificial digestive juices from selected fruiting bodies of edible mushrooms (Boletus badius, Boletus edulis, Cantharellus cibarius, Leccinum scabrum, Pleurotus ostreatus, Suillus bovinus) before and after thermal processing which imitated food preparation. The total amount of zinc released from thermally-processed mushrooms ranged within 2.22–20.68 mg/100 g dry weight. The highest amount of zinc was determined in artificial digestive juices in thermally-processed fruiting bodies of B. badius and B. edulis. For C. cibarius fruiting bodies, thermal processing resulted in a slight increase in the release of zinc compared to the unprocessed fruiting bodies.