Nanoparticle tracking and analysis measurements were
performed with a NanoSight LM20 instrument, equipped
with a sample chamber with a 640 nm laser and a Viton
fluoroelastomer O-ring. The sample was injected into the
sample chamber with the help of sterile syringes until the
liquid sample reached to the tip of the nozzle. The measurements
were made at room temperature. The software
used for capturing and analyzing the data was the NTA 2.1
Build 127.