, with some
modifications. The first process of this experiment is the fruit
wounding process. The citrus fruits were wounded with a sterile
borer at two points (4 mm deep 4 mm wide) at the equator of
each fruit. The next process is the pathogen inoculation process. In
this stage, each wound was inoculated with 20 mL of
1 104 spores mL1 suspension of P. italicum or P. digitatum. After
drying, these fruits were averagely divided into four groups for
contrast. Group 1 received HWT, which means this group was
immersed in a circulating water bath at 53 C for 2 min before it
was forced-air cooled at 20 C to air dry. Fruits in group 2 were
inoculated aliquots (30 mL) of the cell suspensions of
P. membranaefaciens (1 108 cells mL1
) on each of their wounds.
Group 3 received HWT, and the same yeast cell suspensions in
group 2 were inoculated. Group 4 served as the control group
without any treatment in this stage. In the final stage, all fruits were
packed separately by plastic bags and incubated at 20 C with 85%e
90% relative humidity (RH). The disease incidence and lesion
diameter of each fruit were measured according to the method of
Z
, with somemodifications. The first process of this experiment is the fruitwounding process. The citrus fruits were wounded with a sterileborer at two points (4 mm deep 4 mm wide) at the equator ofeach fruit. The next process is the pathogen inoculation process. Inthis stage, each wound was inoculated with 20 mL of1 104 spores mL1 suspension of P. italicum or P. digitatum. Afterdrying, these fruits were averagely divided into four groups forcontrast. Group 1 received HWT, which means this group wasimmersed in a circulating water bath at 53 C for 2 min before itwas forced-air cooled at 20 C to air dry. Fruits in group 2 wereinoculated aliquots (30 mL) of the cell suspensions ofP. membranaefaciens (1 108 cells mL1) on each of their wounds.Group 3 received HWT, and the same yeast cell suspensions ingroup 2 were inoculated. Group 4 served as the control groupwithout any treatment in this stage. In the final stage, all fruits werepacked separately by plastic bags and incubated at 20 C with 85%e90% relative humidity (RH). The disease incidence and lesiondiameter of each fruit were measured according to the method ofZ
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