of the corrected anisotropy decay of TMA-DPH in the POPC membrane measured at 0.1 MPa and 100 MPa, where the membrane is in the liquid-crystalline and the gel phase, respectively. Fluorescence of TMA-DPH is significantly depolarized at 0.1 MPa while the depolarization
is limited at 100 MPa, reflecting the phases of the POPC membranes [22]. Pressure-induced phase transition obtained using the HP-TRFAM systemis in agreementwith previous spectroscopic and thermodynamic studies [21,23]. HP-TRFAM characterizes the phase transition of the
POPC membrane accompanying steep increases in S at the expected hydrostatic pressures at 10 and 20 °C (Fig. 2). The rotational diffusion coefficient Dw for TMA-DPH is greater at 20 °C than at 10 °C and decreases with pressure in concert with the gradual increase in S in the Lα phase. In the Lβ phase, at pressures above 75 MPa (10 °C) and 100 MPa (20 °C), an exponential approximation of the anisotropy decay with time fails to determine the rotational correlation time for TMA-DPH motion within the highly ordered POPC gel (Fig. 2).
of the corrected anisotropy decay of TMA-DPH in the POPC membrane measured at 0.1 MPa and 100 MPa, where the membrane is in the liquid-crystalline and the gel phase, respectively. Fluorescence of TMA-DPH is significantly depolarized at 0.1 MPa while the depolarization
is limited at 100 MPa, reflecting the phases of the POPC membranes [22]. Pressure-induced phase transition obtained using the HP-TRFAM systemis in agreementwith previous spectroscopic and thermodynamic studies [21,23]. HP-TRFAM characterizes the phase transition of the
POPC membrane accompanying steep increases in S at the expected hydrostatic pressures at 10 and 20 °C (Fig. 2). The rotational diffusion coefficient Dw for TMA-DPH is greater at 20 °C than at 10 °C and decreases with pressure in concert with the gradual increase in S in the Lα phase. In the Lβ phase, at pressures above 75 MPa (10 °C) and 100 MPa (20 °C), an exponential approximation of the anisotropy decay with time fails to determine the rotational correlation time for TMA-DPH motion within the highly ordered POPC gel (Fig. 2).
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