the blood samples are then put inside 3 ml canisters that already have blood anticoagulants (EDTA). next the samples are put inside Esky (coolers) to be taken to a laboratory to have the blood cholesterol profile examined (triglycerides, HDL LDL and cholesterol by using an enzyme method (CHOD-PAP). meanwhile the liebermann-Burchard method is used to analyze meat and egg cholesterol