We evaluated the
efficacy of Pluronic-85 as an adjuvant to enhance OMVs
protection against B. melitensis. Results showed that Pluronic-
85 enhanced the secretion of TNF-α in macrophages
after incubation with a mixture of OMVs and Pluronic-85.
Also, a combination of OMVs and Pluronic-85 showed better
protection in mice against B. melitensis challenge than
OMVs alone (Jain-Gupta et al. 2012). Thus, OMVs could
be the basis for the development of safer Brucella vaccine
including a human vaccine that is currently not available.