One limitation of the present study is that different depots of AT
were used to measure adipocyte hypertrophy and gene expression
studies (epididymal) and arachidonic acid and protein analysis
(retroperitoneal). This was due to the fact that the epididymal
depot was exhausted in preparing the SVF faction and histological
sections. It is possible that differences in inflammatory response exist
between different AT depots. Such questions will need to be
investigated in future studies