FFA composition was analysed using a GC/MS system (Agilent
6980N series, Agilent Tech. Inc., Santa Clara, CA, USA). Operating
conditions for the GC were as follows: flame ionization detector
(Agilent Tech. Inc.) at 200 C; column: DB-23
(60 m 0.25 mm 0.25 mm) held for 15 min at 165 C, then
T. Erkaya et al. / International Dairy Journal 49 (2015) 102e110 103
increased to 200 C over 47 min; flow gas: H2 (34 kPa); flow rate of
1 mL min1; time stable: 200; flow speed: 1/50 (hydrogen/dry air);
air flow: 350 mL min1; H2 flow: 35mL min1. The FFA composition
analysis was carried out in triplicate.