Plating and the evaluation of the suspected colonies;after incubation, serial dilution of the enrichment culture was prepared using peptone water for each sample. 0.1 ml aliquot was aseptically transferred from the dilution of each sample unto the sterile petridishes containing Aeromonas agar to which Aeromonas selective supplement (AES80004) was added and plated out using the spread plate method. The plates were incubated at 37oC for 24 h. At the end of the incubation period of 24 h, the plates were observed for bacterial growth and representative colonies (green colonies with opaque centre and yellow colonies were randomly selected). Isolates were sub-cultured on nutrient agar and incubated at 37oC for 24 h. The colonies were then tested for Gram stain, oxidase, catalase, motility, growth without sodium chloride, growth in 6% sodium chloride
and growth at 37oC.