Inoculum preparation for leaf bioassay
An Alternariasolaniisolate was obtained from infected tomato leaves in cultivar Arkavikas, IIHR, Bangalore, it was
propagated on potato dextrose agar (PDA) in 90-mm-diameter petri dishes and in 10 days of incubation the culture
covered the entire plate as a matte. The culture was sub-cultured on PDA 3 – 4 days prior to use, plugs of
Alternariasolani were formed with a sterile #1 cork borer (~ 3 mm diameter) around the perimeter of actively growing
culture just before inoculation.