2.3.2. Gas chromatography analysis/mass spectrometry analysis
For GC/MS detection, an electron ionization system, with ionization
energy of 70 eV, a scan time of 1.5 s and mass range
40–300 amu, was used. Helium was the carrier gas at a flow
rate of 1.2 mL/min. Injector and transfer line temperatures
were set at 250 and 280 C, respectively. Oven program
temperature was the same with GC analysis. Diluted samples
(1/10 in hexane, v/v) of 1.0 lL were injected manually and in
the splitless mode. The identification of the compounds was
based on mass spectra (compared with Wiley 275.L, 6th edition
mass spectral library) or with authentic compounds and
confirmed by comparison of their retention indices either with
those of authentic compounds or with data published in the literature
as described by Adams (2001). Further confirmation
was done from Retention Index data generated from a series
of n-alkanes retention indices (relative to C9–C28 on the HP-
5 MS capillary column).
2.3.3. Seed germination and seedling
2.3.2. Gas chromatography analysis/mass spectrometry analysisFor GC/MS detection, an electron ionization system, with ionizationenergy of 70 eV, a scan time of 1.5 s and mass range40–300 amu, was used. Helium was the carrier gas at a flowrate of 1.2 mL/min. Injector and transfer line temperatureswere set at 250 and 280 C, respectively. Oven programtemperature was the same with GC analysis. Diluted samples(1/10 in hexane, v/v) of 1.0 lL were injected manually and inthe splitless mode. The identification of the compounds wasbased on mass spectra (compared with Wiley 275.L, 6th editionmass spectral library) or with authentic compounds andconfirmed by comparison of their retention indices either withthose of authentic compounds or with data published in the literatureas described by Adams (2001). Further confirmationwas done from Retention Index data generated from a seriesof n-alkanes retention indices (relative to C9–C28 on the HP-5 MS capillary column).2.3.3. Seed germination and seedling
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