Determination of pectinase activity
A UVeVis spectrophotometric dinitrosalicylic acid reagent
(DNS) based method was adopted to determine the pectin
lyase activity (Miller, 1959). All of the recorded enzyme activities
were expressed as U/mL while, a unit of pectinase activity
was defined as a total amount of enzyme required to release
1 mmol of galacturonic acid per mL/min under standard assay
conditions.