CONCLUSIONS
6.1 Micropropagation of Rhynchostylis gigantea (Amethyst-purple) 6.1.1 Modified VW liquid medium supplemented with 10 g/L sucrose and 150 ml/L coconut water showed the most suitable for induction of PLB formation
from shoot tips of R. gigantea (Amethyst-purple).
6.1.2 The suitable sucrose concentration used for shoot tip culture
was 10 g/L.
6.1.3 The highest PLB formation (90.3 PLBs) was observed on
modified VW agar medium supplemented with 2 mg/L BA in combination with 1 mg/L NAA.
6.1.4 The highest number of shoots was observed on SH agar medium supplemented with 0.1 mg/L TDZ (73.3 shoots per explant).
6.1.5 The highest shoot produced roots (51%) and number of roots (2.3 roots/ explant) were observed on SH agar medium supplemented with 1.0 mg/L NAA and 1 g/L activated charcoal.
6.1.6 There was no difference in morphology and similar patterns of ploidy levels were observed by flow cytometry from the leaves of both stock plants and regenerated plants with PGRs were supplemented in the medium.
6.1.7 Survival rate of plantlets cultured on charcoal chip in greenhouse for 3 months was 100%.
From this study, we propose the micropropagation process of R. gigantea (Lindl.) Ridl. (Amethyst-purple) in figure 6.1.