PVC columns with 16 cm diameter and 50 cm height were used in the experiment. Each column was packed first with 4.5 kg subsurface soil and 4.5 kg surface soil. The surface soil was mixed with the basal fertilizers as designed before packing. Before transplanting,all PVC column pots were irrigated three days for pre-incubation. Twenty-five-day rice seedlings were transplanted at the center of the pot on July 3,with each pot containing two seedlings. The rice plants in each pot were harvested on October 15, 2009. After harvest, the dry weights of straw and grain yield were measured. The concentration of N in the plant samples was determined using the Kjedahl method.