were subsequently
infiltrated with increasing ratios of Spurr’s resin over 72 h
(Spurr’s resin: DEE, 2:8 for 24 h, 5:5 for 24 h, and 8:2 for 24 h) before
finally being infiltrated with 100% Spurr’s resin overnight for 12 h.
Once substitution was complete samples were embedded in fresh
resin and cured at 60 ◦C for 24 h. Approximately 6–10m sections
were cut using a dry glass knife microtome (Leica Microsystems),
and then mounted onto standard ANSTO aluminium holders ready
for analysis