Methanol and 70% ethanol were the efficient solvents for extracting didehydrostemofoline from the roots with nearly the same yields of 0.393 ±0.007 and 0.388± 0.001% w/w, respectively. Seventy percent ethanol, which is less hazardous than methanol, was recommended as the solvent for extracting S. collinsiae roots. Comparative analysis of didehydrostemofoline
in the root extracts prepared by different extraction methods (sonication, reflux, Soxhlet, maceration,
percolation) with 70% ethanol showed that reflux and sonication gave the highest content of didehydrostemofoline.