The formation of microbial biofilm is a very complex process. Firstly, organic molecules from food are deposited on the surfaces of equipments. Secondly, biologically active microorganisms are attracted to the conditioned surfaces. Thirdly, some microbial cells remain even after cleaning and sanitizing, and initiate growth. Lastly, larger biofilms are formed with the help of expression of genes and quorum sensing (Fig. 1). In the process of biofilm formation, properties of substratum and cell surfaces, surrounding environmental factors and genetic regulation of bacteria play an important role in reversible or irreversible attachment, micro-colony formation to a large biofilm.