In the present study, the effects of antimony on Oryzias latipes (Japanese medaka), Moina macrocopa (crustacea), Simocephalus mixtus (crustacea), and Pseudokirchneriella subcapitata (green algae) were evaluated to investigate the adverse effect of antimony in aquatic environment. Embryos and larvae of Oryzias latipes were used to reduce sacrifice of adult vertebrates as alternative methods. Antimony in the form of APT was used due to its high water solubility relative to other antimony compounds such as antimony chloride (SbCl3) and sodium antimonite (NaSbO3). To the best of our knowledge, this study is the first to use Oryzias latipes, Moina macrocopa, S. mixtus and Pseudokirchneriella subcapitata to assess the effect of APT.