The outcome of the technical improvisation of the
existing technique could be evaluated in terms of cost (volumetric reduction of reagents used)
and time effectiveness (total estimation time). However, the chemical requirement was the
same in both cases. As can be summarized from Table 1, there was an almost fourfold decrease
in the total time of estimation in the improvised technique. Practically, there was no drastic
change in the FA concentration determined following both procedures.