When the embryogenic calli were transferred into the four nutrient media containing
(2 mg/L IAA+ 2 mg/L TDZ) and 100 mg/L ABA, these further developed into cotyledonary embryos.
Maximum number of somatic embryos (2.9 embryos per leaf explant, with a frequency 46.6%) was
obtained in C. oliveri ssp. Oliveri. During subculture using the half strength media, cotyledonary embryos
gradually developed into plantlets.