RESULTS
Comparison of concentration of bioethanol was shown in Figure 1. Fermentation at temperature 35°C showed the
highest concentration of bioethanol, compared with fermentation at 30 and 23°C with reading of 6.21, 5.88
and 5.39% (v/v), respectively. The concentration of bioethanol increased as the temperatures increased
(Figure 1).Reading of the total soluble solid and pH measurement of banana mash treated with different temperature were shown in Table 1. Total soluble solid of the banana mash before fermentation were slightly higher than those after fermentation. The highest total soluble solid was occupied by banana mash treated at temperature 23°
followed by 30 and 35°C. The pH measurements of banana mash before fermentation were higher than pH
measurement of fermented mash after fermentation. The concentration, total soluble solid values and pH of
bioethanol can be considered as significantly difference based on ANOVA method at p < 0.05.
The concentration of bioethanol at different shaking hour was shown in Figure 2. The fermented banana
mash that have been shaken for a long time produced higher bioethanol with 6.55% (v/v), followed by 3 h of
shaking period (6.35%) and fermentation of banana mash without shaking, only produce 5.86% of bioethanol. The
concentration of bioethanol increased as the time of the shaking process was increased. Based on the data, the
values of the total soluble solid for the fermented banana mash were lower than before fermentation (Table 2).
From the pH results, measurements of the pH before fermentation were higher than after fermentation. The pH
of the fermented banana mash without shaking exhibited the lower value than those that have been shake for 3
and 6 h. There were significant differences in this parameter in the concentration of bioethanol and there
was no significant difference on pH measurement between fermented banana shake with 3 and 6 h. The
result of the bioethanol produced from fermented banana mash showed that the concentration of bioethanol
produced range from 4.33 to 6.36% (Figure 3). The lowest volume of 4.33% (v/v) was produced from the
fermented banana mash without water, while the highest volume of 6.36% (v/v) produced from the fermented
banana mash treated with 35% of water. The fermented banana mash treated with 15 and 25% of water,
produced 5.37 and 5.86% of bioethanol concentration,