Also included in the analysis were H. vesicula and H. tartarea .
Sequences were aligned using MAFFT , v6 using the Q-INS-i option .
Afterwards sequences were concatenated for phylogenetic inference.
To ensure reproducibility and to avoid subjective biases, no manual editing except for the removal of leading and trailing gaps was done.
Minimum evolution trees were computed using MEGA v4.0 phylogenetic analysis software, with factory settings, except for using the Tamura-Neimodel of nucleotide substitution