Saccharification was performed in 250-mL reactors in a
Tornado IS6 Overhead Stirring System (Radleys Discovery
Technologies, Shire Hill, Saffron Walden, UK) equipped
with an anchored stirring shaft
using fiber dispersed in
35 mL of 0.05 M sodium acetate buffer (pH 5.0) to which
distilled water was added to reach 1% glucan loading