Genetic mapping of Mt-NS1
Genetic mapping of Mt-NS1 was initially carried out in an F2 population derived from the cross between A20 and F83005.5, which allowed us to map the Mt-NS1 locus on chromosome 8, defined by the flanking markers MtB112 and 40H12L (Figure 3B). However, the ex- tremely low levels of sequence polymorphisms between A20 and F83005.5 prevented us from using this popula- tion for fine mapping of the locus. To solve this prob- lem, we developed another F2 population derived from the cross of DZA045.5 (Nod + Fix+) and F83005.5.