fed through the top of the column. The eluted oil was kept chilled unit the stripping procedure was complete, and then the oil was dried under rotary evaporation using an R-215 rotary evaporator(Buchi, Newcastle, Del., U.AS.A.) WITH A V-855 vacuum controller.
The oil was further stored under high vacuum for 4 h to remove trace amounts of hexane.
The sttipped oil was checked by HPLC to verify that tocopherols had beed adequately removed during oil stripping (Winkler-Moser and others 2014)