sulfanilamide and anti-β-actin antibody were purchased
from the Sigma Chemical Co. (St. Louis,MO).Dimethyl sulfoxide
(DMSO) was purchased from the Merck Co. (Darmstadt, Germany).
Dulbecco’smodified Eagle’smedium(DMEM), fetal bovine serum(FBS),
L-glutamine and the antibiotic mixture (penicillin-streptomycin) were
purchased fromthe Invitrogen Co. (Carlsbad,CA).Anti-COX-2 and antiiNOS
antibodies were purchased from ABcam (Cambridge, MA). Antirabbit
and anti-mouse secondary horseradish peroxidase conjugated
antibodies were purchased from Bethyl Laboratories (Montgomery,
TX). Protein molecular mass markers were obtained from Pharmacia
Biotech (Saclay, France). Polyvinylidene fluoride (PVDF) membranes for
Western blotting were obtained fromMillipore (Bedford, MA). All other
chemicals were reagent grade.
Sample Preparation. A 20 g