Laparoscopic artificial insemination (LAI): All does
submitted to the operation were restricted from feed
for 24 hours and water for 12 hours (Mobini et al.,
2012). They were intravenously anesthetized with
xylazine HCl (0.1 mg/kg) and ketamine HCl (4.4
mg/kg). The analgesic drug (0.04 mg/kg
phenylbutazone) and antibiotic (20,000 IU/kg
penicillin-streptomycin) were also given
intramuscularly. The does were positioned on a
trolley in dorsal recumbency with the head tilted
down at about 45° angle. After ovarian observation to
confirm the presence of graafian follicles, post-thaw
sperm (60 x 106 spermatozoa in 0.25 ml) were
inseminated into the lumen of each uterine horn at
halfway between the utero-tubular junction (UTJ) and
the bifurcation (Ritar et al., 1990).