10 g of pork sample were asepticall

10 g of pork sample were aseptically placed into a stomacher
bag. After that it was homogenized with 90 mL of sterile 0.1%
peptone water in a masticator blender for 3 min at room temperature.
For each sample, appropriate serial decimal dilutions were
prepared in sterile saline and triplicate 0.1 mL sample of appropriate
dilution was poured or spread on total count and selective
agar plates. Total viable counts were enumerated in Plate Count
Agar (PCA, Base Bio-Tech, Hangzhou, China) after incubation at
30
C for 48 h. Lactic acid bacteria were determined on Man Rogosa
Sharpe Agar (Base Bio-Tech, Hangzhou, China) after incubation at
30
C for 5 days. Enterobacteriaceae was determined using Violet
Red Bile Glucose Agar (Base Bio-Tech, Hangzhou, China) incubated
at 37
C for 24 h. Pseudomonas spp. were determined on Pseudomonas
Selective Agar (Base Bio-Tech, Hangzhou, China) with
Pseudomonas CFC Selective Supplement (Base Bio-Tech, Hangzhou,
China) previously ready and incubated at 25
C for 48 h. Microbial
colonies were counted and expressed as lg cfu (colony forming
units) per gram of pork meat (Lorenzo, Batlle, & G
omez, 2014).