Determine how the valves should be arranged and connect with appropriate lengths of teflon tubing. Leave one outlet
port/valve. Determine the length of the teflon tubing pieces needed to reach from the valves (when mounted) to the
enrichment containers. Cut one piece/valve and attach to each outlet port. Place 2" long pieces of 3/16" aquarium tubing over
the end of each outlet valve tubing piece. This enables insertion of 1 ml pipets. Mount the assembled bubbler unit to a board
placed at the back of the water bath or on a rack suspended over the bath. It can also be mounted in a 42°C air incubator with
a shaking platform placed inside and the gas line connected through the wall of the incubator. A second line should go to a
bubbler in a 37°C incubator.