Neurological function test
Neurological/behavioral measurements were performed before MCAO and at 1, 3, 7, 10, 14, 21 and 28 days after intravenous transplantation by an investigator who was blinded to the experimental groups. A set of the Neurobiological Severity Scores (NSS) modified form previous report was used to evaluate sensorimotor behavior including 5 raising the tail tests, 4 placed on the floor tests, 3 sensory tests, and coordination and balance behavior including 3 beam tests [17]. In the severity scores of injury, 1 score point is awarded for the inability to perform the test or for the lack of a tested reflex. Thus the higher the score the more severe is the injury. Rats typically had average testing scores between 8-12 prior to cell transplantation in our experiment.
Neurological function testNeurological/behavioral measurements were performed before MCAO and at 1, 3, 7, 10, 14, 21 and 28 days after intravenous transplantation by an investigator who was blinded to the experimental groups. A set of the Neurobiological Severity Scores (NSS) modified form previous report was used to evaluate sensorimotor behavior including 5 raising the tail tests, 4 placed on the floor tests, 3 sensory tests, and coordination and balance behavior including 3 beam tests [17]. In the severity scores of injury, 1 score point is awarded for the inability to perform the test or for the lack of a tested reflex. Thus the higher the score the more severe is the injury. Rats typically had average testing scores between 8-12 prior to cell transplantation in our experiment.
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