The cells were acquired with an LSR Fortessa flow cytometer (BD Biosciences), and at least 30 000 CD3+ T-cells were analysed with FACSDiva software (BD Biosciences). IA was studied according to the single and double expression of HLA-DR and CD38 in T-cell subsets, grouped as na€ıve (CD45RA +CD45RO), effector memory (CD45RACD45RO+CCR7+) and central memory (TCM; CD45RACD45RO+CCR7), as previously described [21,22].