DPPH radical scavenging activity was assessed according to Kikuzaki et al. (2002). In this assay, a-50 μl of extract or fraction solutions with different concentrations was added with 1.0 ml of 0.4 mM methanolic-DPPH and added with methanol to 5.0 ml. The mixture was shaken vigorously using vortex and left to stand for 30 min at room temperature in a dark room. The scavenging effect on the DPPH radical was read using spectrophotometer (Genesys10) at 517 nm.