Fig. 5. Immunological analysis of recombinant proteins against allergic patients sera and non- allergic sera. The allergic serum pool was pre-incubated with E. coli extract
(A). Then two blots were prepared, one was incubated with the pre-incubated allergic sera pool (B) and the other was incubated with non-allergic sera pool (C). All blots
were then incubated with anti-human IgE secondary antibody and developed with a chromogenic substrate.