In the lab, 5 plates were immediately treated for
quantification of spore biomass (see Experimental
procedure 2.3). Depending on the observed germlings
growth, each experiment lasted for 10–14 days wherein each plate was placed separately in 500 ml of seawater collected in a beach near the Mondego estuary —
Figueira da Foz, previously filtered (Whatman GF/F),
and enriched with PES — Provasoli Enriched Seawater
(modification of L. Provasoli, 1963, in Bold andWynne,
1978)