Thirty specific antibodies negative (SAN) chickens were divided
into two groups consisting of 15 in treatment group and 15 age
matched unvaccinated chickens in control group. The treatment
group was vaccinated with 0.3 ml of a commercial inactivated
(FAdV-4) oil adjuvanted vaccine at the age of 10 days, subcutaneously
at the back of neck. Ten sera sample each were collected
randomly at zero day of vaccination (10 days old chickens) from
both vaccinated (before vaccination) and control groups. Further
10 sera samples were collected at random from each of both vaccinated
and control groups at 5th, 6th, 7th and 8th weeks old age