Abstract
The purpose of this research is to separate the bacteria that produce lactic acid and observe the characteristics of bacteria that produce lactic acid by using the wood to paste on healthy Thai volunteer in Thai Buddhist community around Rajapat Baan Somdej Chaophraya University. The bacteria that produce lactic acid is separated from MRS agar. The different bacteria could be separated totally 56 isolates from 74 volunteers. Bacteria colony could be classified into 3 types which are Small, Medium, and Large, having the cloudy white color, smooth edge, and embossed. After studied the pH value in liquid food, the value from bacteria was between 4-6. When studied the bacteria characteristics by Gram coloring, the bacteria with positive Gram have 2 characterisrics which are rod and cocci. The bacteria separated could be used for other characteristics testing in the future.