Electropherograms were edited in Chromas v1.45 (Techne-
lysium Pty., Tewantin, Australia). Alignment was done by eye in
Bioedit v7.0.1 (Hall, 1999). Indels were coded with the “simple
indel coding” method of Simmons and Ochoterena (2000).
There is no reason to suspect incongruence from a uniparentally
inherited, non-recombining genome, therefore the different gene
regions were immediately combined for phylogenetic analysis.
Parsimony analyses were conducted using PAUP⁎4.0b10
(Swofford, 2003). Heuristic searches were done on a combined
dataset. One thousand replicates of random stepwise taxon
addition were performed, holding one tree at each step with TBR
branch swapping saving no more than 100 trees per replicate.
This was followed by a second round of TBR branch swapping
using all trees retained in memory. Support for each node was
inferred using the bootstrap procedure (Felsenstein, 1985).
Bootstrapping was performed using NoNa (Goloboff, 1993),
spawned as a daughter process from WinClada (Nixon, 2002).
One thousand bootstrap replicates were run with 100 TBR
searches per replicate, holding 10 trees per replicate, followed by
“max⁎” to swap to completion.