In conclusion, a protocol involved exogenous parameters for
the micropropagation of G. changii was optimized based on daily
growth rate of explants. The culture conditions for G. changii
were found to be optimal in 25% PES medium supplemented with
5 mg L−1 AMPEP and salinity of 30 ppt. The protein profile revealed
that no stress induced proteins were produced during in vitro
culture condition. The present study will enhance the clonal propagation
of high quality seedlings which will lead to an increased
production in agar.