Genetic typing of D. hansenii
Minisatellite M13 PCR amplification of 187 D. hansenii isolates
generated 12 different electrophoretic patterns (Fig. 2) labeled A1
to A5, B1 to B5, C1 and D1. The most abundant patternwas A1 (48%)
constituted by 6 bands, the heaviest band at 1500 bp and the
smallest at 400 bp. Pattern B1 displayed the largest number of
bands, 11, the top band at 1400 bp and the bottom one around
300 bp. Patterns A2 to A5 and B2 to B5 displayed minor differences
respect to A1 and B1, respectively; these pattern differences, which
consist of few additional or absent bands are indicated with arrows
on Fig. 2.