Our study sought to understand how past, low atmospheric CO
2
concentrations ([CO
]) impact respiration (R) of soybean (Glycine max), when compared to plants grown under current and future [CO
]s. Experiments were conducted using plants grown under 290, 400 and 700 ppm [CO
]. Leaf R was measured in both darkness (R
D
) and in the light (R
; using the Kok method), with short-term changes in measurement [CO
2
] and [O
2
L
] being used to explore the relationship between light inhibition of leaf R and photorespiration. Root R, photosynthesis (A), leaf [N] and biomass allocation traits were also quantified. In contrast to the inhibitory effect of low growth [CO
2
] on A, growth [CO
] had no significant effect on leaf R
D
or root R. Irrespective of growth [CO
2
], R
L
was always lower than R
D
2
, with light inhibiting leaf R by 17–47%. Importantly, the degree of light inhibition of leaf R was lowest in plants grown under low [CO
], with variations in R
L
being positively correlated with R
and photorespiration. Irrespective of whether leaf R was measured in the light or dark, a greater proportion of the carbon fixed by leaf photosynthesis was released by leaf R in plants grown under low [CO
2
D
] than under current/future [CO
]’s. Collectively, our results highlight the differential responses of A and R to growth of plants under low to elevated atmospheric [CO
2
2
]. © 2014 Elsevier Ireland Ltd. All rights reserved.
2
2
2
2
Our study sought to understand how past, low atmospheric CO2concentrations ([CO]) impact respiration (R) of soybean (Glycine max), when compared to plants grown under current and future [CO]s. Experiments were conducted using plants grown under 290, 400 and 700 ppm [CO]. Leaf R was measured in both darkness (RD) and in the light (R; using the Kok method), with short-term changes in measurement [CO2] and [O2L] being used to explore the relationship between light inhibition of leaf R and photorespiration. Root R, photosynthesis (A), leaf [N] and biomass allocation traits were also quantified. In contrast to the inhibitory effect of low growth [CO2] on A, growth [CO] had no significant effect on leaf RDor root R. Irrespective of growth [CO2], RLwas always lower than RD2, with light inhibiting leaf R by 17–47%. Importantly, the degree of light inhibition of leaf R was lowest in plants grown under low [CO], with variations in RLbeing positively correlated with Rand photorespiration. Irrespective of whether leaf R was measured in the light or dark, a greater proportion of the carbon fixed by leaf photosynthesis was released by leaf R in plants grown under low [CO2D] than under current/future [CO]’s. Collectively, our results highlight the differential responses of A and R to growth of plants under low to elevated atmospheric [CO22]. © 2014 Elsevier Ireland Ltd. All rights reserved.2222
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Our study sought to understand how past, low atmospheric CO
2
concentrations ([CO
]) impact respiration (R) of soybean (Glycine max), when compared to plants grown under current and future [CO
]s. Experiments were conducted using plants grown under 290, 400 and 700 ppm [CO
]. Leaf R was measured in both darkness (R
D
) and in the light (R
; using the Kok method), with short-term changes in measurement [CO
2
] and [O
2
L
] being used to explore the relationship between light inhibition of leaf R and photorespiration. Root R, photosynthesis (A), leaf [N] and biomass allocation traits were also quantified. In contrast to the inhibitory effect of low growth [CO
2
] on A, growth [CO
] had no significant effect on leaf R
D
or root R. Irrespective of growth [CO
2
], R
L
was always lower than R
D
2
, with light inhibiting leaf R by 17–47%. Importantly, the degree of light inhibition of leaf R was lowest in plants grown under low [CO
], with variations in R
L
being positively correlated with R
and photorespiration. Irrespective of whether leaf R was measured in the light or dark, a greater proportion of the carbon fixed by leaf photosynthesis was released by leaf R in plants grown under low [CO
2
D
] than under current/future [CO
]’s. Collectively, our results highlight the differential responses of A and R to growth of plants under low to elevated atmospheric [CO
2
2
]. © 2014 Elsevier Ireland Ltd. All rights reserved.
2
2
2
2
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