A newly introduced sulphydryl compound, dithiothreitol (DTT), is evaluated for its
optimal conditions of inactivation of IgM antibodies. The maximal effects of DTT reagent are
observed when its final concentrations are between 00025 M and 0005 M, pH between 7 0 and 8O0,
and incubation at 370C. Concentrations over 0 01 M, pH values greater than 8-0, and an incubation
temperature over 40'C resulted in a gel formation of the specimen. Examination of both 'cold' and
'warm' type antibodies demonstrated that the results obtained by the DTT reagent are in close
agreement with those obtained by 2-mercaptoethanol reagents and DEAE Sephadex treatment.
Since the procedure is simple and rapid and lacks offensive odour, DTT is recommended for routine
use in blood banking for the inactivation of IgM antibodies.
A newly introduced sulphydryl compound, dithiothreitol (DTT), is evaluated for itsoptimal conditions of inactivation of IgM antibodies. The maximal effects of DTT reagent areobserved when its final concentrations are between 00025 M and 0005 M, pH between 7 0 and 8O0,and incubation at 370C. Concentrations over 0 01 M, pH values greater than 8-0, and an incubationtemperature over 40'C resulted in a gel formation of the specimen. Examination of both 'cold' and'warm' type antibodies demonstrated that the results obtained by the DTT reagent are in closeagreement with those obtained by 2-mercaptoethanol reagents and DEAE Sephadex treatment.Since the procedure is simple and rapid and lacks offensive odour, DTT is recommended for routineuse in blood banking for the inactivation of IgM antibodies.
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