Preoptic area transcript abundances of CRF and CRF-BP were not
significantly affected by the stressor after 1 h recovery. CRF mRNA
levels were significantly reduced by mifepristone treatment in unstressed
fish (0.090 ± 0.036 vs 0.025 ± 0.005, respectively; Fig. 3A).
While this difference was no longer significant at 1 h post-stress,
the overall effect of the mifepristone diet was a significant 2.5-fold
reduction in POA CRFmRNA (p < 0.05). Conversely, CRF-BP transcript
abundance in the POA was not affected by diet (Fig. 3B; p > 0.05).